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High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas

BACKGROUND: Previous studies on high grade sarcomas using mass spectrometry imaging showed proteasome activator complex subunit 1 (PSME1) to be associated with poor survival in soft tissue sarcoma patients. PSME1 is involved in immunoproteasome assembly for generating tumor antigens presented by MHC...

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Autores principales: Lou, Sha, Cleven, Arjen H. G., Balluff, Benjamin, de Graaff, Marieke, Kostine, Marie, Briaire-de Bruijn, Inge, McDonnell, Liam A., Bovée, Judith V. M. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045577/
https://www.ncbi.nlm.nih.gov/pubmed/27733900
http://dx.doi.org/10.1186/s13569-016-0057-z
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author Lou, Sha
Cleven, Arjen H. G.
Balluff, Benjamin
de Graaff, Marieke
Kostine, Marie
Briaire-de Bruijn, Inge
McDonnell, Liam A.
Bovée, Judith V. M. G.
author_facet Lou, Sha
Cleven, Arjen H. G.
Balluff, Benjamin
de Graaff, Marieke
Kostine, Marie
Briaire-de Bruijn, Inge
McDonnell, Liam A.
Bovée, Judith V. M. G.
author_sort Lou, Sha
collection PubMed
description BACKGROUND: Previous studies on high grade sarcomas using mass spectrometry imaging showed proteasome activator complex subunit 1 (PSME1) to be associated with poor survival in soft tissue sarcoma patients. PSME1 is involved in immunoproteasome assembly for generating tumor antigens presented by MHC class I molecules. In this study, we aimed to validate PSME1 as a prognostic biomarker in an independent and larger series of soft tissue sarcomas by immunohistochemistry. METHODS: Tissue microarrays containing leiomyosarcomas (n = 34), myxofibrosarcomas (n = 14), undifferentiated pleomorphic sarcomas (n = 15), undifferentiated spindle cell sarcomas (n = 4), pleomorphic liposarcomas (n = 4), pleomorphic rhabdomyosarcomas (n = 2), and uterine leiomyomas (n = 7) were analyzed for protein expression of PSME1 using immunohistochemistry. Survival times were compared between high and low expression groups using Kaplan–Meier analysis. Cox regression models as multivariate analysis were performed to evaluate whether the associations were independent of other important clinical covariates. RESULTS: PSME1 expression was variable among soft tissue sarcomas. In leiomyosarcomas, high expression was associated with overall poor survival (p = 0.034), decreased metastasis-free survival (p = 0.002) and lower event-free survival (p = 0.007). Using multivariate analysis, the association between PSME1 expression and metastasis-free survival was still significant (p = 0.025) and independent of the histological grade. CONCLUSIONS: High expression of PSME1 is associated with poor metastasis-free survival in soft tissue leiomyosarcoma patients, and might be used as an independent prognostic biomarker. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13569-016-0057-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-50455772016-10-12 High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas Lou, Sha Cleven, Arjen H. G. Balluff, Benjamin de Graaff, Marieke Kostine, Marie Briaire-de Bruijn, Inge McDonnell, Liam A. Bovée, Judith V. M. G. Clin Sarcoma Res Research BACKGROUND: Previous studies on high grade sarcomas using mass spectrometry imaging showed proteasome activator complex subunit 1 (PSME1) to be associated with poor survival in soft tissue sarcoma patients. PSME1 is involved in immunoproteasome assembly for generating tumor antigens presented by MHC class I molecules. In this study, we aimed to validate PSME1 as a prognostic biomarker in an independent and larger series of soft tissue sarcomas by immunohistochemistry. METHODS: Tissue microarrays containing leiomyosarcomas (n = 34), myxofibrosarcomas (n = 14), undifferentiated pleomorphic sarcomas (n = 15), undifferentiated spindle cell sarcomas (n = 4), pleomorphic liposarcomas (n = 4), pleomorphic rhabdomyosarcomas (n = 2), and uterine leiomyomas (n = 7) were analyzed for protein expression of PSME1 using immunohistochemistry. Survival times were compared between high and low expression groups using Kaplan–Meier analysis. Cox regression models as multivariate analysis were performed to evaluate whether the associations were independent of other important clinical covariates. RESULTS: PSME1 expression was variable among soft tissue sarcomas. In leiomyosarcomas, high expression was associated with overall poor survival (p = 0.034), decreased metastasis-free survival (p = 0.002) and lower event-free survival (p = 0.007). Using multivariate analysis, the association between PSME1 expression and metastasis-free survival was still significant (p = 0.025) and independent of the histological grade. CONCLUSIONS: High expression of PSME1 is associated with poor metastasis-free survival in soft tissue leiomyosarcoma patients, and might be used as an independent prognostic biomarker. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13569-016-0057-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-10-01 /pmc/articles/PMC5045577/ /pubmed/27733900 http://dx.doi.org/10.1186/s13569-016-0057-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Lou, Sha
Cleven, Arjen H. G.
Balluff, Benjamin
de Graaff, Marieke
Kostine, Marie
Briaire-de Bruijn, Inge
McDonnell, Liam A.
Bovée, Judith V. M. G.
High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title_full High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title_fullStr High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title_full_unstemmed High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title_short High nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
title_sort high nuclear expression of proteasome activator complex subunit 1 predicts poor survival in soft tissue leiomyosarcomas
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045577/
https://www.ncbi.nlm.nih.gov/pubmed/27733900
http://dx.doi.org/10.1186/s13569-016-0057-z
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