Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

BACKGROUND: Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional transl...

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Detalles Bibliográficos
Autores principales: Wandrey, Georg, Wurzel, Joel, Hoffmann, Kyra, Ladner, Tobias, Büchs, Jochen, Meinel, Lorenz, Lühmann, Tessa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045631/
https://www.ncbi.nlm.nih.gov/pubmed/27733867
http://dx.doi.org/10.1186/s13036-016-0031-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045631/
https://www.ncbi.nlm.nih.gov/pubmed/27733867
http://dx.doi.org/10.1186/s13036-016-0031-6

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