Cargando…

Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

BACKGROUND: Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional transl...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wandrey, Georg, Wurzel, Joel, Hoffmann, Kyra, Ladner, Tobias, Büchs, Jochen, Meinel, Lorenz, Lühmann, Tessa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045631/ https://www.ncbi.nlm.nih.gov/pubmed/27733867 http://dx.doi.org/10.1186/s13036-016-0031-6

Ejemplares similares

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system
por: Wandrey, Georg, et al.
Publicado: (2016)

Editorial: Expansion of the Genetic Code: Unnatural Amino Acids and their Applications
por: Bag, Subhendu Sekhar, et al.
Publicado: (2022)

Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture
por: Ladner, Tobias, et al.
Publicado: (2017)

Probing the molecular basis of hERG drug block with unnatural amino acids
por: Macdonald, Logan C., et al.
Publicado: (2018)

Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids
por: Villa, Jordan K., et al.
Publicado: (2017)

Spotting priming-active compounds using parsley cell cultures in microtiter plates
por: Hoffmann, Kyra, et al.
Publicado: (2023)

Expansion of the Genetic Alphabet: Unnatural Nucleobases and Their Applications
por: Bag, Subhendu Sekhar, et al.
Publicado: (2012)

Unnatural amino acids: better than the real things?
por: Minnihan, Ellen C, et al.
Publicado: (2009)

Exploring the role of unnatural amino acids in antimicrobial peptides
por: Oliva, Rosario, et al.
Publicado: (2018)

Synthesis of complex unnatural fluorine-containing amino acids
por: Brittain, William D.G., et al.
Publicado: (2020)

Reprogramming natural proteins using unnatural amino acids
por: Adhikari, Anup, et al.
Publicado: (2021)

Using Unnatural Amino Acids to Probe the Energetics of Oxyanion Hole Hydrogen Bonds in the Ketosteroid Isomerase Active Site
por: Natarajan, Aditya, et al.
Publicado: (2014)

Fluorescent probing for RNA molecules by an unnatural base-pair system
por: Kimoto, Michiko, et al.
Publicado: (2007)

Switching DNA-binding specificity by unnatural amino acid substitution
por: Maiti, Atanu, et al.
Publicado: (2005)

Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis
por: Wang, Feng, et al.
Publicado: (2010)

Site-Specific Labeling of Proteins Using Unnatural Amino Acids
por: Lee, Kyung Jin, et al.
Publicado: (2019)

Study and design of amino acid-based radical enzymes using unnatural amino acids
por: Yuan, Feiyan, et al.
Publicado: (2023)

An Expanded Set of Amino Acid Analogs for the Ribosomal Translation of Unnatural Peptides
por: Hartman, Matthew C. T., et al.
Publicado: (2007)

Scaleable catalytic asymmetric Strecker syntheses of unnatural α-amino acids
por: Zuend, Stephan J., et al.
Publicado: (2009)

Synthesis of Unnatural Amino Acids Functionalized with Sterically Shielded Pyrroline Nitroxides
por: Wang, Ying, et al.
Publicado: (2014)

Visible-light photoredox synthesis of unnatural chiral α-amino acids
por: Jiang, Min, et al.
Publicado: (2016)

Design, synthesis and characterization of linear unnatural amino acids for skin moisturization
por: Arezki, N. R., et al.
Publicado: (2016)

Advances and Challenges in Cell-Free Incorporation of Unnatural Amino Acids Into Proteins
por: Gao, Wei, et al.
Publicado: (2019)

An Unnatural Amino Acid-Regulated Growth Controller Based on Informational Disturbance
por: Kato, Yusuke
Publicado: (2021)

Development of mammalian cell logic gates controlled by unnatural amino acids
por: Mills, Emily M., et al.
Publicado: (2021)

Unnatural Amino Acid Crosslinking for Increased Spatiotemporal Resolution of Chromatin Dynamics
por: Moleri, Pamela, et al.
Publicado: (2023)

Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma
por: HIRAO, Ichiro, et al.
Publicado: (2012)

Unnatural causes /
por: James, P. D.
Publicado: (1981)

Unnatural Death
Publicado: (1899)

Cellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression
por: Mühlmann, Martina, et al.
Publicado: (2017)

Conditional knockout tools: Application of site-specific incorporation of unnatural amino acid via genetic code expansion in viral and parasite vaccine development
por: Liu, Pinghua, et al.
Publicado: (2017)

Directed Evolution of a Designer Enzyme Featuring an Unnatural Catalytic Amino Acid
por: Mayer, Clemens, et al.
Publicado: (2019)

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
por: Drienovská, Ivana, et al.
Publicado: (2015)

A Hydroxyquinoline‐Based Unnatural Amino Acid for the Design of Novel Artificial Metalloenzymes
por: Drienovská, Ivana, et al.
Publicado: (2020)

Stereoselective synthesis of unnatural α-amino acid derivatives through photoredox catalysis
por: Shatskiy, Andrey, et al.
Publicado: (2021)

Pyrrolysine-Inspired in Cellulo Synthesis of an Unnatural Amino Acid for Facile Macrocyclization of Proteins
por: Tai, Jingxuan, et al.
Publicado: (2023)

The unnatural nature of science
por: Wolpert, Lewis
Publicado: (1992)

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content
por: Huber, Robert, et al.
Publicado: (2009)

RF1 Knockout Allows Ribosomal Incorporation of Unnatural Amino Acids at Multiple Sites
por: Johnson, David B.F., et al.
Publicado: (2011)

An engineered bacterium auxotrophic for an unnatural amino acid: a novel biological containment system
por: Kato, Yusuke
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_2r5pvj91lbqidjd17ap2q4bccj