A vector platform for the rapid and efficient engineering of stable complex transgenes

We describe the generation of a set of plasmid vector tools that allow the rapid generation of complex-interacting stable transgenes in immortalized and primary cells. Of particular importance is inclusion of a mechanism to monitor the activation status of regulatory pathways via a reporter cassette...

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Detalles Bibliográficos
Autores principales: Jäckel, Carsten, Nogueira, Melanie Schmitt, Ehni, Nadja, Kraus, Christiane, Ranke, Julius, Dohmann, Maike, Noessner, Elfriede, Nelson, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046065/
https://www.ncbi.nlm.nih.gov/pubmed/27694838
http://dx.doi.org/10.1038/srep34365

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046065/
https://www.ncbi.nlm.nih.gov/pubmed/27694838
http://dx.doi.org/10.1038/srep34365

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