Developing a de novo targeted knock-in method based on in utero electroporation into the mammalian brain

Genome-editing technology has revolutionized the field of biology. Here, we report a novel de novo gene-targeting method mediated by in utero electroporation into the developing mammalian brain. Electroporation of donor DNA with the CRISPR/Cas9 system vectors successfully leads to knock-in of the do...

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Detalles Bibliográficos
Autores principales: Tsunekawa, Yuji, Terhune, Raymond Kunikane, Fujita, Ikumi, Shitamukai, Atsunori, Suetsugu, Taeko, Matsuzaki, Fumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Techniques and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047673/
https://www.ncbi.nlm.nih.gov/pubmed/27578183
http://dx.doi.org/10.1242/dev.136325

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047673/
https://www.ncbi.nlm.nih.gov/pubmed/27578183
http://dx.doi.org/10.1242/dev.136325

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