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Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, an...

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Detalles Bibliográficos
Autores principales: Koolivand, Davoud, Bashir, Nemat Sokhandan, Behjatnia, Seyed Aliakbar, Joozani, Raziallah Jafari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051564/
https://www.ncbi.nlm.nih.gov/pubmed/27721695
http://dx.doi.org/10.5423/PPJ.OA.01.2016.0031