Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities

Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve exper...

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Detalles Bibliográficos
Autores principales: Cheng, Fei, Hou, Lin, Woeste, Keith, Shang, Zhengchun, Peng, Xiaobang, Zhao, Peng, Zhang, Shuoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Environmental Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052337/
https://www.ncbi.nlm.nih.gov/pubmed/27528083
http://dx.doi.org/10.1016/j.bjm.2016.06.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052337/
https://www.ncbi.nlm.nih.gov/pubmed/27528083
http://dx.doi.org/10.1016/j.bjm.2016.06.007

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