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Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities
Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve exper...
Autores principales: | Cheng, Fei, Hou, Lin, Woeste, Keith, Shang, Zhengchun, Peng, Xiaobang, Zhao, Peng, Zhang, Shuoxin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052337/ https://www.ncbi.nlm.nih.gov/pubmed/27528083 http://dx.doi.org/10.1016/j.bjm.2016.06.007 |
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