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A Modified Enrichment Method to Construct Microsatellite Library from Plateau Pika Genome (Ochotona curzoniae)

A microsatellite-enriched library of plateau pika (Ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hy...

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Detalles Bibliográficos
Autores principales: Geng, Jianing, Li, Kexin, Zhang, Yanming, Hu, Songnian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054129/
https://www.ncbi.nlm.nih.gov/pubmed/20451163
http://dx.doi.org/10.1016/S1672-0229(10)60007-1