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A Modified Enrichment Method to Construct Microsatellite Library from Plateau Pika Genome (Ochotona curzoniae)
A microsatellite-enriched library of plateau pika (Ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hy...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054129/ https://www.ncbi.nlm.nih.gov/pubmed/20451163 http://dx.doi.org/10.1016/S1672-0229(10)60007-1 |