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Cell surface GRP78 as a biomarker and target for suppressing glioma cells
High-grade glioma is a highly malignant and metastatic brain cancer, resistant to many existing anticancer treatments. In such glioma cancer cells, the glucose-regulated protein 78 kDa (GRP78) is particularly highly up-regulated. Former studies have thus targeted mutation-free GRP78 not only to dete...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054676/ https://www.ncbi.nlm.nih.gov/pubmed/27713511 http://dx.doi.org/10.1038/srep34922 |
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author | Kang, Bo Ram Yang, Seung-Hoon Chung, Bo-Ryehn Kim, Woong Kim, YoungSoo |
author_facet | Kang, Bo Ram Yang, Seung-Hoon Chung, Bo-Ryehn Kim, Woong Kim, YoungSoo |
author_sort | Kang, Bo Ram |
collection | PubMed |
description | High-grade glioma is a highly malignant and metastatic brain cancer, resistant to many existing anticancer treatments. In such glioma cancer cells, the glucose-regulated protein 78 kDa (GRP78) is particularly highly up-regulated. Former studies have thus targeted mutation-free GRP78 not only to detect glioma cancer cells specifically but also to enhance cytotoxic effect. We focus on cell surface-expressed GRP78 as a target for suppressing high-grade glioma cell lines. Glioblastoma multiforme (GBM) cell line, highly malignant glioma cells, was first injected into 5-week-old athymic mice to confirm and compare GRP78 expression in vivo in xenografted and normal brain tissue. Immunofluorescence and immunoblotting were utilized to detect surface-localized GRP78 in diverse high-grade glioma cell lines. By treating glioma cell lines with the polyclonal N-20 antibody against surface-localized GRP78, we subsequently studied the significance of surface GRP78 to the survival and growth of the glioma cell lines. We found that inhibiting the function of surface GRP78 suppressed cancer cell survival and growth proving that the surface-expressed GRP78 is a vital receptor involved in the proliferation of high-grade glioma. Our findings provide opportunities to target surface GRP78 as a biomarker for high-grade glioma and to develop effective cell-specific anticancer therapy. |
format | Online Article Text |
id | pubmed-5054676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50546762016-10-19 Cell surface GRP78 as a biomarker and target for suppressing glioma cells Kang, Bo Ram Yang, Seung-Hoon Chung, Bo-Ryehn Kim, Woong Kim, YoungSoo Sci Rep Article High-grade glioma is a highly malignant and metastatic brain cancer, resistant to many existing anticancer treatments. In such glioma cancer cells, the glucose-regulated protein 78 kDa (GRP78) is particularly highly up-regulated. Former studies have thus targeted mutation-free GRP78 not only to detect glioma cancer cells specifically but also to enhance cytotoxic effect. We focus on cell surface-expressed GRP78 as a target for suppressing high-grade glioma cell lines. Glioblastoma multiforme (GBM) cell line, highly malignant glioma cells, was first injected into 5-week-old athymic mice to confirm and compare GRP78 expression in vivo in xenografted and normal brain tissue. Immunofluorescence and immunoblotting were utilized to detect surface-localized GRP78 in diverse high-grade glioma cell lines. By treating glioma cell lines with the polyclonal N-20 antibody against surface-localized GRP78, we subsequently studied the significance of surface GRP78 to the survival and growth of the glioma cell lines. We found that inhibiting the function of surface GRP78 suppressed cancer cell survival and growth proving that the surface-expressed GRP78 is a vital receptor involved in the proliferation of high-grade glioma. Our findings provide opportunities to target surface GRP78 as a biomarker for high-grade glioma and to develop effective cell-specific anticancer therapy. Nature Publishing Group 2016-10-07 /pmc/articles/PMC5054676/ /pubmed/27713511 http://dx.doi.org/10.1038/srep34922 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kang, Bo Ram Yang, Seung-Hoon Chung, Bo-Ryehn Kim, Woong Kim, YoungSoo Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title | Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title_full | Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title_fullStr | Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title_full_unstemmed | Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title_short | Cell surface GRP78 as a biomarker and target for suppressing glioma cells |
title_sort | cell surface grp78 as a biomarker and target for suppressing glioma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054676/ https://www.ncbi.nlm.nih.gov/pubmed/27713511 http://dx.doi.org/10.1038/srep34922 |
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