Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high resolution cell imaging

Regulatory authorities require that cell lines used in commercial production of recombinant proteins must be derived from a single cell progenitor or clone. The limiting dilution method of cell cloning required multiple rounds of low‐density cell plating and microscopic observation of a single cell...

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Detalles Bibliográficos
Autores principales: Evans, Krista, Albanetti, Thomas, Venkat, Raghavan, Schoner, Ronald, Savery, James, Miro‐Quesada, Guillermo, Rajan, Bhargavi, Groves, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
SPECIAL SECTION: Articles presented at the Cell Culture Engineering XIV (CCE XIV) conference, Québec city, Canada, May 4–9, 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054913/
https://www.ncbi.nlm.nih.gov/pubmed/26195345
http://dx.doi.org/10.1002/btpr.2145

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054913/
https://www.ncbi.nlm.nih.gov/pubmed/26195345
http://dx.doi.org/10.1002/btpr.2145

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