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Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high resolution cell imaging
Regulatory authorities require that cell lines used in commercial production of recombinant proteins must be derived from a single cell progenitor or clone. The limiting dilution method of cell cloning required multiple rounds of low‐density cell plating and microscopic observation of a single cell...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054913/ https://www.ncbi.nlm.nih.gov/pubmed/26195345 http://dx.doi.org/10.1002/btpr.2145 |