Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF(121) Expressed in E. coli Origami B (DE3) with Molecular Chaperones

We describe the production of a highly-active mutant VEGF variant, α(2)-PI(1-8)-VEGF(121), which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α(2)-PI(1-8)-VEGF(121) gene was synthesized, cloned into a pET-32a(+) vector and expr...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaplan, Ondřej, Zárubová, Jana, Mikulová, Barbora, Filová, Elena, Bártová, Jiřina, Bačáková, Lucie, Brynda, Eduard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055331/
https://www.ncbi.nlm.nih.gov/pubmed/27716773
http://dx.doi.org/10.1371/journal.pone.0163697

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055331/
https://www.ncbi.nlm.nih.gov/pubmed/27716773
http://dx.doi.org/10.1371/journal.pone.0163697

Ejemplares similares