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Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF(121) Expressed in E. coli Origami B (DE3) with Molecular Chaperones
We describe the production of a highly-active mutant VEGF variant, α(2)-PI(1-8)-VEGF(121), which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α(2)-PI(1-8)-VEGF(121) gene was synthesized, cloned into a pET-32a(+) vector and expr...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055331/ https://www.ncbi.nlm.nih.gov/pubmed/27716773 http://dx.doi.org/10.1371/journal.pone.0163697 |