HIV-1 Vpr N-terminal tagging affects alternative splicing of the viral genome

To facilitate studies on Vpr function in replicating HIV-1, we aimed to tag the protein in an infectious virus. First we showed that N-, but not C-terminal HA/FLAG tagging of Vpr protein preserves Vpr cytopathicity. Cloning the tags into proviral DNA however ablated viral production and replication....

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Detalles Bibliográficos
Autores principales: Baeyens, Ann, Naessens, Evelien, Van Nuffel, Anouk, Weening, Karin E., Reilly, Anne-Marie, Claeys, Eva, Trypsteen, Wim, Vandekerckhove, Linos, Eyckerman, Sven, Gevaert, Kris, Verhasselt, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056386/
https://www.ncbi.nlm.nih.gov/pubmed/27721439
http://dx.doi.org/10.1038/srep34573

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056386/
https://www.ncbi.nlm.nih.gov/pubmed/27721439
http://dx.doi.org/10.1038/srep34573

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