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Selection of an Appropriate Protein Extraction Method to Study the Phosphoproteome of Maize Photosynthetic Tissue

Often plant tissues are recalcitrant and, due to that, methods relying on protein precipitation, such as TCA/acetone precipitation and phenol extraction, are usually the methods of choice for protein extraction in plant proteomic studies. However, the addition of precipitation steps to protein extra...

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Detalles Bibliográficos
Autores principales: Luís, Inês M., Alexandre, Bruno M., Oliveira, M. Margarida, Abreu, Isabel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058499/
https://www.ncbi.nlm.nih.gov/pubmed/27727304
http://dx.doi.org/10.1371/journal.pone.0164387