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RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining

Background. Proper rRNA depletion is crucial for the successful utilization of FFPE specimens when studying gene expression. We performed a study to evaluate two major rRNA depletion methods: Ribo-Zero and RNase H. RNAs extracted from 4 samples were treated with the two rRNA depletion methods in dup...

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Detalles Bibliográficos
Autores principales: Guo, Yan, Wu, Jie, Zhao, Shilin, Ye, Fei, Su, Yinghao, Clark, Travis, Sheng, Quanhu, Lehmann, Brian, Shu, Xiao-ou, Cai, Qiuyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059559/
https://www.ncbi.nlm.nih.gov/pubmed/27774452
http://dx.doi.org/10.1155/2016/9837310