Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression

Advanced glycation end products (AGEs) are major inflammatory mediators in diabetes, affecting atherosclerosis progression via macrophages. Metformin slows diabetic atherosclerosis progression through mechanisms that remain to be fully elucidated. The present study of murine bone marrow derived macr...

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Autores principales: Zhou, Zhong'e, Tang, Yong, Jin, Xian, Chen, Chengjun, Lu, Yi, Liu, Liang, Shen, Chengxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059570/
https://www.ncbi.nlm.nih.gov/pubmed/27761470
http://dx.doi.org/10.1155/2016/4847812
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author Zhou, Zhong'e
Tang, Yong
Jin, Xian
Chen, Chengjun
Lu, Yi
Liu, Liang
Shen, Chengxing
author_facet Zhou, Zhong'e
Tang, Yong
Jin, Xian
Chen, Chengjun
Lu, Yi
Liu, Liang
Shen, Chengxing
author_sort Zhou, Zhong'e
collection PubMed
description Advanced glycation end products (AGEs) are major inflammatory mediators in diabetes, affecting atherosclerosis progression via macrophages. Metformin slows diabetic atherosclerosis progression through mechanisms that remain to be fully elucidated. The present study of murine bone marrow derived macrophages showed that (1) AGEs enhanced proinflammatory cytokines (interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α)) mRNA expression, RAGE expression, and NFκB activation; (2) metformin pretreatment inhibited AGEs effects and AGEs-induced cluster designation 86 (CD86) (M1 marker) expression, while promoting CD206 (M2 marker) surface expression and anti-inflammatory cytokine (IL-10) mRNA expression; and (3) the AMPK inhibitor, Compound C, attenuated metformin effects. In conclusion, metformin inhibits AGEs-induced inflammatory response in murine macrophages partly through AMPK activation and RAGE/NFκB pathway suppression.
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spelling pubmed-50595702016-10-19 Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression Zhou, Zhong'e Tang, Yong Jin, Xian Chen, Chengjun Lu, Yi Liu, Liang Shen, Chengxing J Diabetes Res Research Article Advanced glycation end products (AGEs) are major inflammatory mediators in diabetes, affecting atherosclerosis progression via macrophages. Metformin slows diabetic atherosclerosis progression through mechanisms that remain to be fully elucidated. The present study of murine bone marrow derived macrophages showed that (1) AGEs enhanced proinflammatory cytokines (interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α)) mRNA expression, RAGE expression, and NFκB activation; (2) metformin pretreatment inhibited AGEs effects and AGEs-induced cluster designation 86 (CD86) (M1 marker) expression, while promoting CD206 (M2 marker) surface expression and anti-inflammatory cytokine (IL-10) mRNA expression; and (3) the AMPK inhibitor, Compound C, attenuated metformin effects. In conclusion, metformin inhibits AGEs-induced inflammatory response in murine macrophages partly through AMPK activation and RAGE/NFκB pathway suppression. Hindawi Publishing Corporation 2016 2016-09-28 /pmc/articles/PMC5059570/ /pubmed/27761470 http://dx.doi.org/10.1155/2016/4847812 Text en Copyright © 2016 Zhong'e Zhou et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Zhong'e
Tang, Yong
Jin, Xian
Chen, Chengjun
Lu, Yi
Liu, Liang
Shen, Chengxing
Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title_full Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title_fullStr Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title_full_unstemmed Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title_short Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression
title_sort metformin inhibits advanced glycation end products-induced inflammatory response in murine macrophages partly through ampk activation and rage/nfκb pathway suppression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059570/
https://www.ncbi.nlm.nih.gov/pubmed/27761470
http://dx.doi.org/10.1155/2016/4847812
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