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In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors

The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (<1h, n = 1261; 1-2h, n = 1773; 2-3h, n = 1647) on in v...

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Detalles Bibliográficos
Autores principales: Olivera, Ramiro, Moro, Lucia Natalia, Jordan, Roberto, Luzzani, Carlos, Miriuka, Santiago, Radrizzani, Martin, Donadeu, F. Xavier, Vichera, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061425/
https://www.ncbi.nlm.nih.gov/pubmed/27732616
http://dx.doi.org/10.1371/journal.pone.0164049