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Extending digital PCR analysis by modelling quantification cycle data

BACKGROUND: Digital PCR (dPCR) is a technique for estimating the concentration of a target nucleic acid by loading a sample into a large number of partitions, amplifying the target and using a fluorescent marker to identify which partitions contain the target. The standard analysis uses only the pro...

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Detalles Bibliográficos
Autores principales: Wilson, Philip J., Ellison, Stephen L. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062887/
https://www.ncbi.nlm.nih.gov/pubmed/27733121
http://dx.doi.org/10.1186/s12859-016-1275-3