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Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression
CRISPR interference (CRISPRi) represents a newly developed tool for targeted gene repression. It has great application potential for studying gene function and mapping gene regulatory elements. However, the optimal parameters for efficient single guide RNA (sgRNA) design for CRISPRi are not fully de...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062975/ https://www.ncbi.nlm.nih.gov/pubmed/27353328 http://dx.doi.org/10.1093/nar/gkw583 |