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Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

CRISPR interference (CRISPRi) represents a newly developed tool for targeted gene repression. It has great application potential for studying gene function and mapping gene regulatory elements. However, the optimal parameters for efficient single guide RNA (sgRNA) design for CRISPRi are not fully de...

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Detalles Bibliográficos
Autores principales:	Radzisheuskaya, Aliaksandra, Shlyueva, Daria, Müller, Iris, Helin, Kristian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062975/ https://www.ncbi.nlm.nih.gov/pubmed/27353328 http://dx.doi.org/10.1093/nar/gkw583

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