Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the gene of interest without disrupting regulatory...

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Detalles Bibliográficos
Autores principales: Landgraf, Dirk, Huh, Dann, Hallacli, Erinc, Lindquist, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063382/
https://www.ncbi.nlm.nih.gov/pubmed/27736907
http://dx.doi.org/10.1371/journal.pone.0163950

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063382/
https://www.ncbi.nlm.nih.gov/pubmed/27736907
http://dx.doi.org/10.1371/journal.pone.0163950

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