Fluorescence-based characterization of non-fluorescent transient states of tryptophan – prospects for protein conformation and interaction studies

Tryptophan fluorescence is extensively used for label-free protein characterization. Here, we show that by analyzing how the average tryptophan fluorescence intensity varies with excitation modulation, kinetics of tryptophan dark transient states can be determined in a simple, robust and reliable ma...

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Detalles Bibliográficos
Autores principales: Hevekerl, Heike, Tornmalm, Johan, Widengren, Jerker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066179/
https://www.ncbi.nlm.nih.gov/pubmed/27748381
http://dx.doi.org/10.1038/srep35052

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066179/
https://www.ncbi.nlm.nih.gov/pubmed/27748381
http://dx.doi.org/10.1038/srep35052

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