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Fluorescence-based characterization of non-fluorescent transient states of tryptophan – prospects for protein conformation and interaction studies
Tryptophan fluorescence is extensively used for label-free protein characterization. Here, we show that by analyzing how the average tryptophan fluorescence intensity varies with excitation modulation, kinetics of tryptophan dark transient states can be determined in a simple, robust and reliable ma...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066179/ https://www.ncbi.nlm.nih.gov/pubmed/27748381 http://dx.doi.org/10.1038/srep35052 |