Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A

The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of...

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Detalles Bibliográficos
Autores principales: Takahashi, Motoko, Wakai, Toshifumi, Hirota, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066236/
https://www.ncbi.nlm.nih.gov/pubmed/27633014
http://dx.doi.org/10.1101/gad.282855.116
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author Takahashi, Motoko
Wakai, Toshifumi
Hirota, Toru
author_facet Takahashi, Motoko
Wakai, Toshifumi
Hirota, Toru
author_sort Takahashi, Motoko
collection PubMed
description The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes.
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spelling pubmed-50662362017-03-01 Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A Takahashi, Motoko Wakai, Toshifumi Hirota, Toru Genes Dev Research Communication The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes. Cold Spring Harbor Laboratory Press 2016-09-01 /pmc/articles/PMC5066236/ /pubmed/27633014 http://dx.doi.org/10.1101/gad.282855.116 Text en © 2016 Takahashi et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Takahashi, Motoko
Wakai, Toshifumi
Hirota, Toru
Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title_full Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title_fullStr Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title_full_unstemmed Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title_short Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
title_sort condensin i-mediated mitotic chromosome assembly requires association with chromokinesin kif4a
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066236/
https://www.ncbi.nlm.nih.gov/pubmed/27633014
http://dx.doi.org/10.1101/gad.282855.116
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