Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM grids Modified with NTA-Graphene Oxide

We report the fabrication of transmission electron microscopy (TEM) grids bearing graphene oxide (GO) sheets that have been modified with N(α), N(α)-dicarboxymethyllysine (NTA) and deactivating agents to block non-selective binding between GO-NTA sheets and non-target proteins. The resulting GO-NTA-...

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Detalles Bibliográficos
Autores principales: Benjamin, Christopher J., Wright, Kyle J., Bolton, Scott C., Hyun, Seok-Hee, Krynski, Kyle, Grover, Mahima, Yu, Guimei, Guo, Fei, Kinzer-Ursem, Tamara L., Jiang, Wen, Thompson, David H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066248/
https://www.ncbi.nlm.nih.gov/pubmed/27748364
http://dx.doi.org/10.1038/srep32500

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066248/
https://www.ncbi.nlm.nih.gov/pubmed/27748364
http://dx.doi.org/10.1038/srep32500

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