A proximity-dependent assay for specific RNA–protein interactions in intact cells

The proximity ligation assay (PLA) is an immune staining method that detects protein–protein interactions in fixed cells. We describe here RNA–PLA, a simple adaptation of this technology that allows the detection of specific RNA–protein interactions in fixed cells by using a DNA oligonucleotide that...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Wei, Xie, Mingyi, Shu, Mei-Di, Steitz, Joan A., DiMaio, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066630/
https://www.ncbi.nlm.nih.gov/pubmed/27659050
http://dx.doi.org/10.1261/rna.058248.116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066630/
https://www.ncbi.nlm.nih.gov/pubmed/27659050
http://dx.doi.org/10.1261/rna.058248.116

Ejemplares similares