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A proximity-dependent assay for specific RNA–protein interactions in intact cells

The proximity ligation assay (PLA) is an immune staining method that detects protein–protein interactions in fixed cells. We describe here RNA–PLA, a simple adaptation of this technology that allows the detection of specific RNA–protein interactions in fixed cells by using a DNA oligonucleotide that...

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Detalles Bibliográficos
Autores principales:	Zhang, Wei, Xie, Mingyi, Shu, Mei-Di, Steitz, Joan A., DiMaio, Daniel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2016
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066630/ https://www.ncbi.nlm.nih.gov/pubmed/27659050 http://dx.doi.org/10.1261/rna.058248.116

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