Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first expansion microscopy method...

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Detalles Bibliográficos
Autores principales: Tillberg, Paul W., Chen, Fei, Piatkevich, Kiryl D., Zhao, Yongxin, Yu, Chih-Chieh (Jay), English, Brian P., Gao, Linyi, Martorell, Anthony, Suk, Ho-Jun, Yoshida, Fumiaki, DeGennaro, Ellen M., Roossien, Douglas H., Gong, Guanyu, Seneviratne, Uthpala, Tannenbaum, Steven R., Desimone, Robert, Cai, Dawen, Boyden, Edward S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068827/
https://www.ncbi.nlm.nih.gov/pubmed/27376584
http://dx.doi.org/10.1038/nbt.3625

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068827/
https://www.ncbi.nlm.nih.gov/pubmed/27376584
http://dx.doi.org/10.1038/nbt.3625

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