Efficient CRISPR-Mediated Post-Transcriptional Gene Silencing in a Hyperthermophilic Archaeon Using Multiplexed crRNA Expression

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-mediated RNA degradation is catalyzed by a type III system in the hyperthermophilic archaeon Sulfolobus solfataricus. Earlier work demonstrated that the system can be engineered to target specifically mRNA of an endogenous host repor...

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Detalles Bibliográficos
Autores principales: Zebec, Ziga, Zink, Isabelle Anna, Kerou, Melina, Schleper, Christa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068938/
https://www.ncbi.nlm.nih.gov/pubmed/27507792
http://dx.doi.org/10.1534/g3.116.032482

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068938/
https://www.ncbi.nlm.nih.gov/pubmed/27507792
http://dx.doi.org/10.1534/g3.116.032482

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