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Efficient CRISPR-Mediated Post-Transcriptional Gene Silencing in a Hyperthermophilic Archaeon Using Multiplexed crRNA Expression

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-mediated RNA degradation is catalyzed by a type III system in the hyperthermophilic archaeon Sulfolobus solfataricus. Earlier work demonstrated that the system can be engineered to target specifically mRNA of an endogenous host repor...

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Detalles Bibliográficos
Autores principales:	Zebec, Ziga, Zink, Isabelle Anna, Kerou, Melina, Schleper, Christa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2016
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5068938/ https://www.ncbi.nlm.nih.gov/pubmed/27507792 http://dx.doi.org/10.1534/g3.116.032482

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