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Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen

Recombinant protein expression often presents a bottleneck for the production of proteins for use in many areas of animal‐cell biotechnology. Difficult‐to‐express proteins require the generation of numerous expression constructs, where popular prokaryotic screening systems often fail to identify exp...

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Detalles Bibliográficos
Autores principales: Bleckmann, Maren, Schmelz, Stefan, Schinkowski, Christian, Scrima, Andrea, van den Heuvel, Joop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069567/
https://www.ncbi.nlm.nih.gov/pubmed/26913471
http://dx.doi.org/10.1002/bit.25956