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Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes
Two photo‐crosslinking biarsenical (CrAsH‐EDT(2))‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069617/ https://www.ncbi.nlm.nih.gov/pubmed/26948688 http://dx.doi.org/10.1002/ardp.201500440 |
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author | Syeda, Shameem S. Rice, Daren Hook, Derek J. Heckert, Leslie L. Georg, Gunda I. |
author_facet | Syeda, Shameem S. Rice, Daren Hook, Derek J. Heckert, Leslie L. Georg, Gunda I. |
author_sort | Syeda, Shameem S. |
collection | PubMed |
description | Two photo‐crosslinking biarsenical (CrAsH‐EDT(2))‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH‐EDT(2) and N (1)‐(4‐azido‐2‐nitrophenyl)hexane‐1,6‐diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3‐related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest. |
format | Online Article Text |
id | pubmed-5069617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50696172016-11-01 Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes Syeda, Shameem S. Rice, Daren Hook, Derek J. Heckert, Leslie L. Georg, Gunda I. Arch Pharm (Weinheim) Full Papers Two photo‐crosslinking biarsenical (CrAsH‐EDT(2))‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH‐EDT(2) and N (1)‐(4‐azido‐2‐nitrophenyl)hexane‐1,6‐diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3‐related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest. John Wiley and Sons Inc. 2016-03-07 2016-04 /pmc/articles/PMC5069617/ /pubmed/26948688 http://dx.doi.org/10.1002/ardp.201500440 Text en © 2016 The Authors. Archiv der Pharmazie Published by Wiley‐VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Full Papers Syeda, Shameem S. Rice, Daren Hook, Derek J. Heckert, Leslie L. Georg, Gunda I. Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title | Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title_full | Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title_fullStr | Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title_full_unstemmed | Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title_short | Synthesis of Arylazide‐ and Diazirine‐Containing CrAsH‐EDT(2) Photoaffinity Probes |
title_sort | synthesis of arylazide‐ and diazirine‐containing crash‐edt(2) photoaffinity probes |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069617/ https://www.ncbi.nlm.nih.gov/pubmed/26948688 http://dx.doi.org/10.1002/ardp.201500440 |
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