Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

Designing primers for PCR-based taxonomic surveys that amplify a broad range of phylotypes in varied community samples is a difficult challenge, and the comparability of data sets amplified with varied primers requires attention. Here, we examined the performance of modified 16S rRNA gene and intern...

Descripción completa

Detalles Bibliográficos
Autores principales: Walters, William, Hyde, Embriette R., Berg-Lyons, Donna, Ackermann, Gail, Humphrey, Greg, Parada, Alma, Gilbert, Jack A., Jansson, Janet K., Caporaso, J. Gregory, Fuhrman, Jed A., Apprill, Amy, Knight, Rob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2015
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069754/
https://www.ncbi.nlm.nih.gov/pubmed/27822518
http://dx.doi.org/10.1128/mSystems.00009-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069754/
https://www.ncbi.nlm.nih.gov/pubmed/27822518
http://dx.doi.org/10.1128/mSystems.00009-15

Ejemplares similares