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Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

Designing primers for PCR-based taxonomic surveys that amplify a broad range of phylotypes in varied community samples is a difficult challenge, and the comparability of data sets amplified with varied primers requires attention. Here, we examined the performance of modified 16S rRNA gene and intern...

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Detalles Bibliográficos
Autores principales:	Walters, William, Hyde, Embriette R., Berg-Lyons, Donna, Ackermann, Gail, Humphrey, Greg, Parada, Alma, Gilbert, Jack A., Jansson, Janet K., Caporaso, J. Gregory, Fuhrman, Jed A., Apprill, Amy, Knight, Rob
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Microbiology 2015
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069754/ https://www.ncbi.nlm.nih.gov/pubmed/27822518 http://dx.doi.org/10.1128/mSystems.00009-15

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