Impact of RNA degradation on fusion detection by RNA-seq

BACKGROUND: RNA-seq is a well-established method for studying the transcriptome. Popular methods for library preparation in RNA-seq such as Illumina TruSeq® RNA v2 kit use a poly-A pulldown strategy. Such methods can cause loss of coverage at the 5′ end of genes, impacting the ability to detect fusi...

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Detalles Bibliográficos
Autores principales: Davila, Jaime I., Fadra, Numrah M., Wang, Xiaoke, McDonald, Amber M., Nair, Asha A., Crusan, Barbara, R., Wu, Xianglin, Blommel, Joseph H., Jen, Jin, Rumilla, Kandelaria M., Jenkins, Robert B., Aypar, Umut, Klee, Eric W., Kipp, Benjamin R., Halling, Kevin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072325/
https://www.ncbi.nlm.nih.gov/pubmed/27765019
http://dx.doi.org/10.1186/s12864-016-3161-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072325/
https://www.ncbi.nlm.nih.gov/pubmed/27765019
http://dx.doi.org/10.1186/s12864-016-3161-9

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