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Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

The fluorescent Ca(2+) sensitive dyes Fura Red (ratiometric) and Fluo-4 (non-ratiometric) are widely utilized for the optical assessment of Ca(2+) fluctuations in vitro as well as in situ. The fluorescent behavior of these dyes is strongly depends on temperature, pH, ionic strength and pressure. It...

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Detalles Bibliográficos
Autores principales: Schneidereit, D., Vass, H., Reischl, B., Allen, R. J., Friedrich, O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072694/
https://www.ncbi.nlm.nih.gov/pubmed/27764134
http://dx.doi.org/10.1371/journal.pone.0164509