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Failure to Genotype: A Cautionary Note on an Elusive loxP Sequence
Here we report on a technical difficulty we encountered while optimizing genotyping strategies to identify mice derived from Exoc3l2(tm1a(KOMP)Wtsi) embryonic stem cells obtained from the Knockout Mouse Project Repository. The Exoc3l2(tm1a(KOMP)Wtsi) construct encodes a “knockout-first” design with...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074582/ https://www.ncbi.nlm.nih.gov/pubmed/27768725 http://dx.doi.org/10.1371/journal.pone.0165012 |