A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis

Cell-free protein synthesis (CFPS) systems are important laboratory tools that are used for various synthetic biology applications. Here, we present a simple and inexpensive laboratory-scale method for preparing a CFPS system from E. coli. The procedure uses basic lab equipment, a minimal set of rea...

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Detalles Bibliográficos
Autores principales: Krinsky, Nitzan, Kaduri, Maya, Shainsky-Roitman, Janna, Goldfeder, Mor, Ivanir, Eran, Benhar, Itai, Shoham, Yuval, Schroeder, Avi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074584/
https://www.ncbi.nlm.nih.gov/pubmed/27768741
http://dx.doi.org/10.1371/journal.pone.0165137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074584/
https://www.ncbi.nlm.nih.gov/pubmed/27768741
http://dx.doi.org/10.1371/journal.pone.0165137

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