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Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest

Fgf10 is necessary for the development of a number of organs that fail to develop or are reduced in size in the null mutant. Here we have knocked out Fgf10 specifically in the neural crest driven by Wnt1cre. The Wnt1creFgf10fl/fl mouse phenocopies many of the null mutant defects, including cleft pal...

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Autores principales: Teshima, Tathyane H. N., Lourenco, Silvia V., Tucker, Abigail S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078472/
https://www.ncbi.nlm.nih.gov/pubmed/27826253
http://dx.doi.org/10.3389/fphys.2016.00488
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author Teshima, Tathyane H. N.
Lourenco, Silvia V.
Tucker, Abigail S.
author_facet Teshima, Tathyane H. N.
Lourenco, Silvia V.
Tucker, Abigail S.
author_sort Teshima, Tathyane H. N.
collection PubMed
description Fgf10 is necessary for the development of a number of organs that fail to develop or are reduced in size in the null mutant. Here we have knocked out Fgf10 specifically in the neural crest driven by Wnt1cre. The Wnt1creFgf10fl/fl mouse phenocopies many of the null mutant defects, including cleft palate, loss of salivary glands, and ocular glands, highlighting the neural crest origin of the Fgf10 expressing mesenchyme surrounding these organs. In contrast tissues such as the limbs and lungs, where Fgf10 is expressed by the surrounding mesoderm, were unaffected, as was the pituitary gland where Fgf10 is expressed by the neuroepithelium. The circumvallate papilla of the tongue formed but was hypoplastic in the conditional and Fgf10 null embryos, suggesting that other sources of FGF can compensate in development of this structure. The tracheal cartilage rings showed normal patterning in the conditional knockout, indicating that the source of Fgf10 for this tissue is mesodermal, which was confirmed using Wnt1cre-dtTom to lineage trace the boundary of the neural crest in this region. The thyroid, thymus, and parathyroid glands surrounding the trachea were present but hypoplastic in the conditional mutant, indicating that a neighboring source of mesodermal Fgf10 might be able to partially compensate for loss of neural crest derived Fgf10.
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spelling pubmed-50784722016-11-08 Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest Teshima, Tathyane H. N. Lourenco, Silvia V. Tucker, Abigail S. Front Physiol Physiology Fgf10 is necessary for the development of a number of organs that fail to develop or are reduced in size in the null mutant. Here we have knocked out Fgf10 specifically in the neural crest driven by Wnt1cre. The Wnt1creFgf10fl/fl mouse phenocopies many of the null mutant defects, including cleft palate, loss of salivary glands, and ocular glands, highlighting the neural crest origin of the Fgf10 expressing mesenchyme surrounding these organs. In contrast tissues such as the limbs and lungs, where Fgf10 is expressed by the surrounding mesoderm, were unaffected, as was the pituitary gland where Fgf10 is expressed by the neuroepithelium. The circumvallate papilla of the tongue formed but was hypoplastic in the conditional and Fgf10 null embryos, suggesting that other sources of FGF can compensate in development of this structure. The tracheal cartilage rings showed normal patterning in the conditional knockout, indicating that the source of Fgf10 for this tissue is mesodermal, which was confirmed using Wnt1cre-dtTom to lineage trace the boundary of the neural crest in this region. The thyroid, thymus, and parathyroid glands surrounding the trachea were present but hypoplastic in the conditional mutant, indicating that a neighboring source of mesodermal Fgf10 might be able to partially compensate for loss of neural crest derived Fgf10. Frontiers Media S.A. 2016-10-25 /pmc/articles/PMC5078472/ /pubmed/27826253 http://dx.doi.org/10.3389/fphys.2016.00488 Text en Copyright © 2016 Teshima, Lourenco and Tucker. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Teshima, Tathyane H. N.
Lourenco, Silvia V.
Tucker, Abigail S.
Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title_full Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title_fullStr Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title_full_unstemmed Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title_short Multiple Cranial Organ Defects after Conditionally Knocking Out Fgf10 in the Neural Crest
title_sort multiple cranial organ defects after conditionally knocking out fgf10 in the neural crest
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078472/
https://www.ncbi.nlm.nih.gov/pubmed/27826253
http://dx.doi.org/10.3389/fphys.2016.00488
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