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Label-free detection of microRNA based on coupling multiple isothermal amplification techniques

MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to initiate s...

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Detalles Bibliográficos
Autores principales: Zheng, Xiangjiang, Niu, Li, Wei, Di, Li, Xuemei, Zhang, Shusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078768/
https://www.ncbi.nlm.nih.gov/pubmed/27777399
http://dx.doi.org/10.1038/srep35982