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Two New FRET Imaging Measures: Linearly Proportional to and Highly Contrasting the Fraction of Active Molecules

We developed two new FRET imaging measures for intramolecular FRET biosensors, called linearly proportional (LP) and highly contrasting (HC) measures, which can be easily calculated by the fluorescence intensities of donor and acceptor as a ratio between their weighted sums. As an alternative to the...

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Detalles Bibliográficos
Autores principales: Yamao, Masataka, Aoki, Kazuhiro, Yukinawa, Naoto, Ishii, Shin, Matsuda, Michiyuki, Naoki, Honda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5079603/
https://www.ncbi.nlm.nih.gov/pubmed/27780260
http://dx.doi.org/10.1371/journal.pone.0164254