Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for genome editing. In this study, using a microinjection-based CRISPR/Cas9 system, we efficiently generated mouse lines carrying mutations at the Irx3 and Irx5 loci, whic...

Descripción completa

Detalles Bibliográficos
Autores principales: HARA, Satoshi, KATO, Tomoko, GOTO, Yuji, KUBOTA, Souichirou, TAMANO, Moe, TERAO, Miho, TAKADA, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2016
Materias:
Technology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081742/
https://www.ncbi.nlm.nih.gov/pubmed/27396308
http://dx.doi.org/10.1262/jrd.2016-058

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081742/
https://www.ncbi.nlm.nih.gov/pubmed/27396308
http://dx.doi.org/10.1262/jrd.2016-058

Ejemplares similares