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Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength

Structured illumination microscopy (SIM) is a wide-field technique in fluorescence microscopy that provides fast data acquisition and two-fold resolution improvement beyond the Abbe limit. We observed a further resolution improvement using the nonlinear emission response of a fluorescent protein. We...

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Detalles Bibliográficos
Autores principales: Lu-Walther, Hui-Wen, Hou, Wenya, Kielhorn, Martin, Arai, Yoshiyuki, Nagai, Takeharu, Kessels, Michael M., Qualmann, Britta, Heintzmann, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082623/
https://www.ncbi.nlm.nih.gov/pubmed/27783656
http://dx.doi.org/10.1371/journal.pone.0165148