Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5084710/ https://www.ncbi.nlm.nih.gov/pubmed/27666746 http://dx.doi.org/10.1016/j.devcel.2016.08.013 |
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author | Bencivenga, Stefano Serrano-Mislata, Antonio Bush, Max Fox, Samantha Sablowski, Robert |
author_facet | Bencivenga, Stefano Serrano-Mislata, Antonio Bush, Max Fox, Samantha Sablowski, Robert |
author_sort | Bencivenga, Stefano |
collection | PubMed |
description | The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities. |
format | Online Article Text |
id | pubmed-5084710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50847102016-11-01 Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis Bencivenga, Stefano Serrano-Mislata, Antonio Bush, Max Fox, Samantha Sablowski, Robert Dev Cell Article The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities. Cell Press 2016-10-24 /pmc/articles/PMC5084710/ /pubmed/27666746 http://dx.doi.org/10.1016/j.devcel.2016.08.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bencivenga, Stefano Serrano-Mislata, Antonio Bush, Max Fox, Samantha Sablowski, Robert Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title | Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title_full | Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title_fullStr | Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title_full_unstemmed | Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title_short | Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis |
title_sort | control of oriented tissue growth through repression of organ boundary genes promotes stem morphogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5084710/ https://www.ncbi.nlm.nih.gov/pubmed/27666746 http://dx.doi.org/10.1016/j.devcel.2016.08.013 |
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