Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis

The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that...

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Autores principales: Bencivenga, Stefano, Serrano-Mislata, Antonio, Bush, Max, Fox, Samantha, Sablowski, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5084710/
https://www.ncbi.nlm.nih.gov/pubmed/27666746
http://dx.doi.org/10.1016/j.devcel.2016.08.013
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author Bencivenga, Stefano
Serrano-Mislata, Antonio
Bush, Max
Fox, Samantha
Sablowski, Robert
author_facet Bencivenga, Stefano
Serrano-Mislata, Antonio
Bush, Max
Fox, Samantha
Sablowski, Robert
author_sort Bencivenga, Stefano
collection PubMed
description The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities.
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spelling pubmed-50847102016-11-01 Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis Bencivenga, Stefano Serrano-Mislata, Antonio Bush, Max Fox, Samantha Sablowski, Robert Dev Cell Article The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities. Cell Press 2016-10-24 /pmc/articles/PMC5084710/ /pubmed/27666746 http://dx.doi.org/10.1016/j.devcel.2016.08.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bencivenga, Stefano
Serrano-Mislata, Antonio
Bush, Max
Fox, Samantha
Sablowski, Robert
Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title_full Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title_fullStr Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title_full_unstemmed Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title_short Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis
title_sort control of oriented tissue growth through repression of organ boundary genes promotes stem morphogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5084710/
https://www.ncbi.nlm.nih.gov/pubmed/27666746
http://dx.doi.org/10.1016/j.devcel.2016.08.013
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