Disruption of Membranes of Extracellular Vesicles Is Necessary for ELISA Determination of Urine AQP2: Proof of Disruption and Epitopes of AQP2 Antibodies

Aquaporin-2 (AQP2) is present in urine extracellular vesicles (EVs) and is a useful biomarker for water balance disorders. We previously found that pre-treatment of urine with alkali/detergent or storage at −25 °C is required for enzyme-linked immunosorbent assay (ELISA) measurement. We speculated t...

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Detalles Bibliográficos
Autores principales: Nameta, Masaaki, Saijo, Yoko, Ohmoto, Yasukazu, Katsuragi, Kiyonori, Yamamoto, Keiko, Yamamoto, Tadashi, Ishibashi, Kenichi, Sasaki, Sei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085667/
https://www.ncbi.nlm.nih.gov/pubmed/27681727
http://dx.doi.org/10.3390/ijms17101634

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5085667/
https://www.ncbi.nlm.nih.gov/pubmed/27681727
http://dx.doi.org/10.3390/ijms17101634

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