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RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population
Previous studies have reported that two single nucleotide polymorphisms (SNPs) in the RANTES gene promoter region, -403G/A and -28C/G, are associated with a slower rate of decline in CD4+ T cell count. In addition, as a ligand of the major HIV coreceptor CCR5, it is known to block HIV-CCR5 interacti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086385/ https://www.ncbi.nlm.nih.gov/pubmed/27821902 http://dx.doi.org/10.1155/2016/4703854 |
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author | Mutuiri, Shem P. M. Kutima, Helen L. Mwapagha, Lamech M. Munyao, James K. Nyamache, Anthony Kebira Wanjiru, Irene Khamadi, Samoel A. |
author_facet | Mutuiri, Shem P. M. Kutima, Helen L. Mwapagha, Lamech M. Munyao, James K. Nyamache, Anthony Kebira Wanjiru, Irene Khamadi, Samoel A. |
author_sort | Mutuiri, Shem P. M. |
collection | PubMed |
description | Previous studies have reported that two single nucleotide polymorphisms (SNPs) in the RANTES gene promoter region, -403G/A and -28C/G, are associated with a slower rate of decline in CD4+ T cell count. In addition, as a ligand of the major HIV coreceptor CCR5, it is known to block HIV-CCR5 interactions in the course of the HIV infection cycle. This study was carried out with the aim of determining the occurrence of single nucleotide polymorphisms (SNPs) -403G > A and -28C > G in the promoter region of RANTES, in a subset of the Kenyan population. Genomic DNA was extracted from peripheral blood monocular cells and used to amplify the RANTES gene region. Restriction fragment length polymorphism was used to determine the genotypes of the RANTES gene. Out of 100 HIV infected individuals, 19% had G1 genotypes (403G/G, 28C/G), 30% (403A/A, 28C/C), and 50% (403G/A, 28C/C), while in healthy blood donors 13% had G4 (403G/A, 28C/C) genotypes, 22% (403A/A, 28C/C), and 54% (403G/A, 28C/C). HIV negative blood donors (54%) had higher risk of alteration to risk of HIV transmission compared to those who were HIV infected (50%). However, the risk to transmission and distribution differences was not significant (P = 0.092). The study showed that RANTES polymorphisms -403 and -28 alleles do exist in the Kenyan population. |
format | Online Article Text |
id | pubmed-5086385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50863852016-11-07 RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population Mutuiri, Shem P. M. Kutima, Helen L. Mwapagha, Lamech M. Munyao, James K. Nyamache, Anthony Kebira Wanjiru, Irene Khamadi, Samoel A. Dis Markers Research Article Previous studies have reported that two single nucleotide polymorphisms (SNPs) in the RANTES gene promoter region, -403G/A and -28C/G, are associated with a slower rate of decline in CD4+ T cell count. In addition, as a ligand of the major HIV coreceptor CCR5, it is known to block HIV-CCR5 interactions in the course of the HIV infection cycle. This study was carried out with the aim of determining the occurrence of single nucleotide polymorphisms (SNPs) -403G > A and -28C > G in the promoter region of RANTES, in a subset of the Kenyan population. Genomic DNA was extracted from peripheral blood monocular cells and used to amplify the RANTES gene region. Restriction fragment length polymorphism was used to determine the genotypes of the RANTES gene. Out of 100 HIV infected individuals, 19% had G1 genotypes (403G/G, 28C/G), 30% (403A/A, 28C/C), and 50% (403G/A, 28C/C), while in healthy blood donors 13% had G4 (403G/A, 28C/C) genotypes, 22% (403A/A, 28C/C), and 54% (403G/A, 28C/C). HIV negative blood donors (54%) had higher risk of alteration to risk of HIV transmission compared to those who were HIV infected (50%). However, the risk to transmission and distribution differences was not significant (P = 0.092). The study showed that RANTES polymorphisms -403 and -28 alleles do exist in the Kenyan population. Hindawi Publishing Corporation 2016 2016-10-16 /pmc/articles/PMC5086385/ /pubmed/27821902 http://dx.doi.org/10.1155/2016/4703854 Text en Copyright © 2016 Shem P. M. Mutuiri et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mutuiri, Shem P. M. Kutima, Helen L. Mwapagha, Lamech M. Munyao, James K. Nyamache, Anthony Kebira Wanjiru, Irene Khamadi, Samoel A. RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title | RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title_full | RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title_fullStr | RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title_full_unstemmed | RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title_short | RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population |
title_sort | rantes gene polymorphisms associated with hiv-1 infections in kenyan population |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5086385/ https://www.ncbi.nlm.nih.gov/pubmed/27821902 http://dx.doi.org/10.1155/2016/4703854 |
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