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IKKε inhibits PKC to promote Fascin-dependent actin bundling

Signaling molecules have pleiotropic functions and are activated by various extracellular stimuli. Protein kinase C (PKC) is activated by diverse receptors, and its dysregulation is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during developmen...

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Autores principales: Otani, Tetsuhisa, Ogura, Yosuke, Misaki, Kazuyo, Maeda, Takuya, Kimpara, Akiyo, Yonemura, Shigenobu, Hayashi, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087637/
https://www.ncbi.nlm.nih.gov/pubmed/27578797
http://dx.doi.org/10.1242/dev.138495
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author Otani, Tetsuhisa
Ogura, Yosuke
Misaki, Kazuyo
Maeda, Takuya
Kimpara, Akiyo
Yonemura, Shigenobu
Hayashi, Shigeo
author_facet Otani, Tetsuhisa
Ogura, Yosuke
Misaki, Kazuyo
Maeda, Takuya
Kimpara, Akiyo
Yonemura, Shigenobu
Hayashi, Shigeo
author_sort Otani, Tetsuhisa
collection PubMed
description Signaling molecules have pleiotropic functions and are activated by various extracellular stimuli. Protein kinase C (PKC) is activated by diverse receptors, and its dysregulation is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during development remains poorly understood. We have previously shown that a protein kinase, IKKε, is active at the growing bristle tip and regulates actin bundle organization during Drosophila bristle morphogenesis. Here, we demonstrate that IKKε regulates the actin bundle localization of a dynamic actin cross-linker, Fascin. IKKε inhibits PKC, thereby protecting Fascin from inhibitory phosphorylation. Excess PKC activation is responsible for the actin bundle defects in IKKε-deficient bristles, whereas PKC is dispensable for bristle morphogenesis in wild-type bristles, indicating that PKC is repressed by IKKε in wild-type bristle cells. These results suggest that IKKε prevents excess activation of PKC during bristle morphogenesis.
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spelling pubmed-50876372016-11-07 IKKε inhibits PKC to promote Fascin-dependent actin bundling Otani, Tetsuhisa Ogura, Yosuke Misaki, Kazuyo Maeda, Takuya Kimpara, Akiyo Yonemura, Shigenobu Hayashi, Shigeo Development Research Article Signaling molecules have pleiotropic functions and are activated by various extracellular stimuli. Protein kinase C (PKC) is activated by diverse receptors, and its dysregulation is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during development remains poorly understood. We have previously shown that a protein kinase, IKKε, is active at the growing bristle tip and regulates actin bundle organization during Drosophila bristle morphogenesis. Here, we demonstrate that IKKε regulates the actin bundle localization of a dynamic actin cross-linker, Fascin. IKKε inhibits PKC, thereby protecting Fascin from inhibitory phosphorylation. Excess PKC activation is responsible for the actin bundle defects in IKKε-deficient bristles, whereas PKC is dispensable for bristle morphogenesis in wild-type bristles, indicating that PKC is repressed by IKKε in wild-type bristle cells. These results suggest that IKKε prevents excess activation of PKC during bristle morphogenesis. The Company of Biologists Ltd 2016-10-15 /pmc/articles/PMC5087637/ /pubmed/27578797 http://dx.doi.org/10.1242/dev.138495 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Otani, Tetsuhisa
Ogura, Yosuke
Misaki, Kazuyo
Maeda, Takuya
Kimpara, Akiyo
Yonemura, Shigenobu
Hayashi, Shigeo
IKKε inhibits PKC to promote Fascin-dependent actin bundling
title IKKε inhibits PKC to promote Fascin-dependent actin bundling
title_full IKKε inhibits PKC to promote Fascin-dependent actin bundling
title_fullStr IKKε inhibits PKC to promote Fascin-dependent actin bundling
title_full_unstemmed IKKε inhibits PKC to promote Fascin-dependent actin bundling
title_short IKKε inhibits PKC to promote Fascin-dependent actin bundling
title_sort ikkε inhibits pkc to promote fascin-dependent actin bundling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087637/
https://www.ncbi.nlm.nih.gov/pubmed/27578797
http://dx.doi.org/10.1242/dev.138495
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